Fig. 3From: Protein kinase C α enhances migration of breast cancer cells through FOXC2-mediated repression of p120-cateninFOXC2 is a downstream target of PKCα. a Breast cancer cells were treated with either DMSO or TPA (100 nM, 2 h) and FOXC2 expression levels were determined by qRT-PCR. b FOXC2 expression upon PKCα knockdown was assessed at both the transcript and protein level. Blots are representative of three independent replicates. β-actin was used as the loading control. Densitometry analysis of FOXC2 is shown. Graphs represent the SEM of at least three independent biological replicates. Significance was determined by student t-test and two-way ANOVA, followed by Tukey’s tests. *, P < 0.05 **, P < 0.01 ****, P < 0.0001Back to article page