Fig. 8

The reduced expression of tumor β₂ integrin limits the infiltration of suppressor immune cells at the early stages of liver colonization. a-c The effects of the partial deficiency of β₂ integrin expression on the recruitment of immune cell populations 24 h after i.s. tumor cell inoculation was quantified. Immune cell subsets recruited to the liver were immuhistochemically analyzed by labeling with specific antibodies against CD11b and Ly6G antigens (CD11b⁺Ly6G⁺ cells) (a), and CD8 (CD8⁺ T cells) (b) and CD4 (CD4⁺ T cells) (c) lymphocytic markers. The quantification of CD4⁺, CD8⁺, CD11b⁺ and Ly6G⁺ (Gr1⁺) cell numbers was carried out in 3 different sections per mice, and at least 5 mice per group were used per each experiment and each one was performed 3 times. Data are mean values ± SD from 10 different fields/100 mm² liver tissue section. Changes were considered statistically significant at *p < 0.05