Fig. 4
Reduced β2 integrin expression reduces migratory potential of C26 cells through LSEC, and adhesion to and migration through collagen type I. The ability of tumor C26 and β2-C26 cells to migrate across LSEC monolayers (a), and the ability of C26 cells or β2-C26 cells (left) and that of C26, a pool of β2-C26 cells or C26 pre-treated with a neutralizing antibody for β2 integrin (right) to adhere to a layer of collagen type I (b), and to migrate through collagen type I (c) was quantified. Transmigration and migration studies were carried out in modified Boyden chambers. Representative pictures of C26 cells transmigrated through the LSEC are shown in a. Migrated cells are expressed as the average number of cells that migrated per 20× field. Data are mean values ± SD from three different experiments. Differences were considered statistically significant at *p < 0.05 and *p < 0.01