Fig. 3

Modulation of the adhesive potential of C26 cells by reduced expression of β₂ integrin. The ability to adhere to liver endothelial cells (LSECs) of C26 and β₂- C26 cells was tested with adhesion assays. a C26 and β₂-C26 cells were added to cultures of primary isolated LSECs and allow to adhere for 30 min before quantification of cell adhesion. b In subsequent experiments, C26 cells were pre-activated with sICAM-1 (200 ng/ml). Before sICAM-1 activation some tumor cells were pre-treated with neutralizing antibodies for the subunit α_L of the integrin. c In order to rule out other adhesion molecules present on the surface of tumor cells or LSECs, C26 cells were pre-treated either with CD11b or with CD11b/c, and LSECs were pre-treated with VCAM-1 antibodies before the addition of tumor cells to LSEC cultures. Data are mean values ± SD from three different experiments. Differences were considered statistically significant at *p < 0.05 and **p < 0.01