Fig. 7

Verification of the results obtained by proteome profiler analysis. Levels of pAKT, pCREB, pFAK and pMAPK were determined via Western blot analysis. Equal loading of the lanes was confirmed by the detection of α-tubulin for pCREB and pMAPK and β-actin for pFAK and pAKT. The depicted results are representative of three independent experiments. P-values at significance levels of ≤0.050 and ≤0.010 are indicated by (*) and (**), respectively. Comparisons that are both significant and relevant for the definition of responder and non-responder cell lines are marked with black bars and asterisks, whereas comparisons that are only significant are marked with gray bars and asterisks. The average phosphorylation levels were quantified using densitometric analysis and calculated in relation to the levels of the loading control (+SD). Statistical p-values are listed in Additional file 1: Table S7. Additionally, one representative Western blot of each experiment is shown