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Fig. 4 | BMC Cancer

Fig. 4

From: CCL5-CCR5 interactions modulate metabolic events during tumor onset to promote tumorigenesis

Fig. 4

In vitro, MDA-MB-231.CCR5−/− cells are less metabolically active than MDA-MB-231.CCR5+/+ cells. a Cells were either left untreated (medium alone), treated with 10 nM CCL5, or pre-treated with 2 mM 2-DG for 1 h prior to CCL5 treatment, or maintained in medium containing 5 mM glutamine. For all, medium was changed every other day and the treatment(s) reapplied. Cell proliferation was quantified using an MTT assay as described in Methods. The proliferation index is normalized against untreated conditions (ie medium alone). Values are means ± SEM of triplicate assays and each data point combines the data from 3 independent experiments. Statistical analysis was performed comparing untreated cells with CCL5-treated cells and inhibitor-treated cells with CCL5 + inhibitor treated cells, or comparing CCL5-treated with CCL5 + inhibitor-treated cells. b CCL5 levels were measured from culture supernatants after 16 h incubation. c Glucose uptake, d GLUT-1 expression, e intracellular ATP and f intracellular lactate were measured as described in Methods in cells treated with 10 nM CCL5 or 2 μM oligomycin for 3 h. Data are expressed as percent-change relative to untreated MDA-MB-231.CCR5+/+ cells. Values are means ± SEM of triplicate assays and each data point combines the data from 3 independent experiments. * p < 0.05, ** p < 0.01, *** p < 0.001

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