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Fig. 7 | BMC Cancer

Fig. 7

From: Isolation and characterization of a new naturally immortalized human breast carcinoma cell line, KAIMRC1

Fig. 7

a Analysis of drug sensitivity of KAIMRC1 cells. Doxorubicin hydrochloride and Lapatinib were chosen to test drug sensitivity of KAIMRC1 cells in comparison to MDA MB-231 and MCF-7. MTT assay was performed to assess cytotoxicity and cell viability after drug treatment. Drug treatment was performed for 24 h prior to MTT assay in complete media. All three cell lines showed almost the same response to doxorubicin however they responded differently to lapatininb treatment. This result was later validated in breast cancer gene expression analysis. KAIMRC1 cells seems to be the most sensitive among the three cell lines with an IC50 of 5.175e-006 M. X-axis = Relative light unit (RLU) and y-axis = Logarithm of the drug molarity (Log[drug]M). b Gene expression analysis of human breast cells in response to drug treatment. Breast cancer cells KAIMRC-1 were treated with 10 μM of Letrozole, Doxorubicin hydrochloride and Lapatinib with and without serum in complete media. Cells were harvested after 6 h, RNA was isolated and cDNA was synthesized. Real time qPCR was performed using MAPKinase and AKT pathway associated genes to study gene expression changes in response to drug treatment. KAIMRC1 cells were found to be more responsive to drug treatment in comparison to other established cell lines. Treatment of KAIMRC1 cells with Lapatinib showed down regulation of EGFR, MCL1 and RXRα genes which explains the sensitivity of these cells to Lapatinib shown in (b). Letrozole and Doxorubicin showed no difference as compared to other cell lines. X-axis = Drugs and y-axis = Relative gene expression (ΔΔCt) to DMSO control. n = 3 in all cases

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