Fig. 2

a Growth Characteristics of KAIMRC1 cells. The growth kinetics of KAIMRC1 cells at early passages were studied in comparison with other established breast cancer cell lines, MCF-7, MDA MB-231 and normal breast cell line MCF-10A. The average growth curves of the cell lines are shown. All the cells were seeded at 3000 cells per well in a 48-Well plate and counted in triplicates for 24 days. The population-doubling time of MDA MB-231, MCF-7, MCF-10A and KAIMRC1 cells were approximately 45, 24, 18 and 24 h, respectively. b The wound healing assay of KAIMRC1 cells was performed on all the four cell lines. The graph reveals that the proliferation and wound healing ability of KAIMRC1 cells was comparable to other breast cancer cell lines, MCF-7 and MDA MB-231. c Time-lapse images of the wound healing assay performed on the cells. A scratch was made on the confluent layer of the cells (area between the two parallel red lines) and transmitted light time-lapse imaging was performed for 2 days. Images show cells after 0 h, 4 h, 8 h and 20 h. Scale bar = 200 μm