Fig. 5
Effects of VP16 on the expression of P53, P21, Puma, Bcl-2, PARP, cleaved-PARP, caspase3 and cleaved-caspases3 in K562, K562-iASPPhi and K562-Douhi cells. a-c. K562, K562-iASPPhi and K562-Douhi cells were treated with 0, 1, 2, 5, 10 and 20 μg/ml of VP16 respectively for 24 h, then the cells were lysed for Western blot analysis. d-f. K562, K562-iASPPhi and K562-Douhi cells were treated with 5 μg/ml of VP16 for 0, 3, 6, 9, 12 and 24 h, respectively, then cells were lysed for Western blot analysis. Actin was used for a loading control. C-PARP represents cleaved-PARP, C-caspase3 represents cleaved-caspase3