Fig. 3
The biological function of iASPPhi, Sertad1hi and Douhi in K562 cells. a The protein expression level of iASPP and sertad1 gene in vector, iASPPhi, Sertad1hi and Douhi cells. The actin protein was included as a loading control. b The cell proliferation of three groups cells from 0 h to 72 h. c-d The cell proliferation of three groups after treatment with 0.5 μg/ml and 4.0 μg/ml of etoposide (VP16) for 48 h and 72 h, respectively. e-f The early apoptosis percentage of three groups after treatment with 1 μg/ml, 2 μg/ml, 3 μg/ml and 5 μg/ml VP16 for 48 h and 72 h, respectively. Early apoptosis represents the population of AnnexinV+PI−(the apoptosis rate of baseline was below 5%) (* represent P < 0.05).All data are representive of 5 independent experiments