Fig. 2

Interaction between iASPP and Sertad1. a 293, K562 and HL60 cells were lysed by RIPA lysis buffer, antibody against iASPP and Sertad1 were used for IP-antibody, anti-iASPP antibody was used for Western blot. Input was set as positive control and rabbit IgG antibody for negative control. b Co-immunoprecipitation between iASPP and Sertad1 was done in KG1a and U937 cell lines. Antibody against Sertad1 was used for IP, anti-iASPP and anti-Sertad1 were used for IB. c Confocal microscopy images of co-localization of iASPP and Sertad1 in K562, HL60, Nalm6 and HEK293 cells by immunostained with antibodies against iASPP(red) and, Sertad1(green), and cell nucleus stained with DAPI (blue). The maximum resolution of the images in K562, HL60 and Nalm6 cells was 8 μm. The maximum resolution of 293 cells was 20 μm. d The full-length and different domains of Sertad1 with myc tag were constructed and cotransfected with pcDNA3.1-iASPP into 293 cells. Cell lysates were immunoprecipitated with anti-myc antibody and blotted with anti-iASPP antibody