Fig. 1

Detection of iASPP, sertad1 and p53 expression in transcriptional and translational level. a Expression of iASPP mRNA in 293 T and multiple leukemic cell lines. b Expression of sertad1 mRNA in 293 T and multiple leukemic cell lines. c Expression of p53 mRNA in 293 T and multiple leukemic cell lines. d Expression of iASPP, sertad1 and p53 protein in 293 T and multiple leukemic cell lines. The actin protein was included as a loading control. e Expression of sertad1 and p53 mRNA in 4 normal donors (unselected whole bone marrow cells) and 8 primary acute myeloid leukemia patients (the median of the blasts percentage was 82%). GAPDH mRNA was included as control. f Expression of iASPP, sertad1 and p53 protein in normal donors(unselected whole bone marrow cells) and primary acute myeloid leukemia patients. N represent normal donor, P represents de novo AML patients. All data are representive of 3 independent experiments