Table 1 Summary of series of experiments for establishment of an orthotopic bladder cancer model in mice
| Â | Experimental number | |||||
|---|---|---|---|---|---|---|
1 | 2 | 3 | 4 | 5 | 6 | |
Mouse strain | NMRI nude | NMRI nude | A: BALB/c nude B: SCID-beige | A: BALB/c nude B: SCID-beige | SCID-beige | SCID-beige |
Mice used (n) | 12 | 18 | 20 | 20 | 20 | 16 |
Anaesthesia-related deaths (n) | 2 | 0 | 1 | 1 | 1 | 0 |
Mice per treatment arm (n) | A: 5 B: 5 | A: 9 B: 9 | A: 10 B: 9 | A: 10 B: 9 | A: 9 B: 10 | A: 8 B: 8 |
Cell count | 2.0 × 106 | 2.0 × 106 | 2.0 × 106 | A: 5.0 × 10 6 B: 1.0 × 10 6 | A: 0.5 × 10 6 B: 1.0 × 10 6 | 0.5 × 106 |
Dwell time | 2Â h | 2Â h | 2Â h | 2Â h | 1Â h | 30Â min |
Pretreatment of bladder | A: trypsin a B: PLL a | A: PLL B: PLL a | PLL | PLL | PLL | A: PLL B: PLL b |
Tumor cell engraftment | A: 2/5 (40%) B: 2/5 (40%) | A: 2/9 (22%) B: 3/9 (33%) | A: 7/10 (70%) B: 9/9 (100%) | A: 9/10 (90%) B: 9/9 (100%) | A: 9/9 (100%) B: 10/10 (100%) | A: 7/8 (88%) B: 8/8 (100%) |
Signal start (d) | 17.0 ± 2.0 7.5 ± 4.5 | 12.5 ± 5.5 19.7 ± 2.9 | 33.0 ± 14.3 16.7 ± 4.3 | 33.9 ± 18.3 14.8 ± 2.1 | 25.8 ± 3.5 22.4 ± 5.6 | 22.4 ± 2.9 19.4 ± 2.9 |
Signal duration (d) | 18.0 ± 2.0 20.0 ± 3.0 | 12.0 ± 1.0 16.0 ± 6.7 | 14.0 ± 4.0 8.7 ± 3.0 | 10.6 ± 4.0 10.4 ± 1.4 | 13.3 ± 6.1 11.5 ± 4.6 | 19.6 ± 8.2 18.1 ± 8.9 |