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Table 1 Summary of series of experiments for establishment of an orthotopic bladder cancer model in mice

From: An orthotopic xenograft model for high-risk non-muscle invasive bladder cancer in mice: influence of mouse strain, tumor cell count, dwell time and bladder pretreatment

  Experimental number
1 2 3 4 5 6
Mouse strain NMRI nude NMRI nude A: BALB/c nude
B: SCID-beige
A: BALB/c nude
B: SCID-beige
SCID-beige SCID-beige
Mice used (n) 12 18 20 20 20 16
Anaesthesia-related deaths (n) 2 0 1 1 1 0
Mice per treatment arm (n) A: 5
B: 5
A: 9
B: 9
A: 10
B: 9
A: 10
B: 9
A: 9
B: 10
A: 8
B: 8
Cell count 2.0 × 106 2.0 × 106 2.0 × 106 A: 5.0 × 10 6
B: 1.0 × 10 6
A: 0.5 × 10 6
B: 1.0 × 10 6
0.5 × 106
Dwell time 2 h 2 h 2 h 2 h 1 h 30 min
Pretreatment of bladder A: trypsin a
B: PLL a
A: PLL
B: PLL a
PLL PLL PLL A: PLL
B: PLL b
Tumor cell engraftment A: 2/5 (40%)
B: 2/5 (40%)
A: 2/9 (22%)
B: 3/9 (33%)
A: 7/10 (70%)
B: 9/9 (100%)
A: 9/10 (90%)
B: 9/9 (100%)
A: 9/9 (100%)
B: 10/10 (100%)
A: 7/8 (88%)
B: 8/8 (100%)
Signal start (d) 17.0 ± 2.0
7.5 ± 4.5
12.5 ± 5.5
19.7 ± 2.9
33.0 ± 14.3
16.7 ± 4.3
33.9 ± 18.3
14.8 ± 2.1
25.8 ± 3.5
22.4 ± 5.6
22.4 ± 2.9
19.4 ± 2.9
Signal duration (d) 18.0 ± 2.0
20.0 ± 3.0
12.0 ± 1.0
16.0 ± 6.7
14.0 ± 4.0
8.7 ± 3.0
10.6 ± 4.0
10.4 ± 1.4
13.3 ± 6.1
11.5 ± 4.6
19.6 ± 8.2
18.1 ± 8.9
  1. Bold statements highlight the parameters that were varied in the experiment. Signal start and signal duration are shown as mean ± mean deviation
  2. Abbreviation: PLL poly-L-lysine
  3. a Induction of lesions in the urothel by carefully scratching with the cannula of the permanent venous catheters
  4. b To avoid air bubbles in the bladder the catheter was filled with cell suspension prior to catheterization