Fig. 3

Single-cell sequencing showed more subtle differences than multi-region WES. a Clustered heatmap of 24 single tumor cells with SCNA profiles in patient 1 based on Euclidean distance and ward.D method. The x axis was plotted by chromosomes from chr1 to chrX/Y and the y axis was the population labeled by blue. b Clustered heat map and PCA of 35 single tumor cells of patient 2 based on SCNA profiles. Single tumor cells were grouped into two clusters. The x axis was plotted by chromosomes from chr1 to chrX/Y and the y axis was subpopulations labeled by blue and green. c Subclonal SCNAs of patients 1 and 2 divided single tumor cells into two subpopulations, which was in accordance with two clusters identified by PCA. The chromosomes (columns) where subclonal SCNAs more than 1.5 Mb located was showed in colors. The two subpopulations (rows) were labeled in colors. d Single tumor cells showed more differences in regional level than gDNA in reigon A of patient 1. P1: patient1; P2: patient 2