Fig. 2

Single-cell sequencing showed SCNA-based subpopulations within two rectal tumors. a Cluster analysis of single cells of each patient based on copy number profiles. The cluster was constructed by Euclidean distance and ward.D method. The yellow and the green represented diploid cells and tumor cells with SCNAs, respectively. b The procedure to distinguish between diploid normal and tumor cells. Diploid cells without mutations were considered to be normal cells, while cells with both SCNAs and mutations were considered as tumor cells. c The mutations validated in single tumor cells of patient 1 by Sanger sequencing. The blue, the grey and the white presented the mutations, the absence of mutations and the undetected by PCR, respectively. (§) represented this gene had two separate independent mutations (d) The mutations validated in single tumor cells of patient 2 by Sanger sequencing. SAMD9L(§) had two base substitution TC to AA at chr7: 92,763,288-92,763,289