Table 1 Primers used for PCR, LOH and bisulfite sequencing analysis
Gene | Primer | Sequence (5′ to 3′) |
|---|---|---|
Cav1 | Cav-1S | TCTGGGGCGTCGTGCGCAAA |
Cav-1AS | GAACCTTGATGAAGCCTGTG | |
C1⍺A | AGTTTTCATCCAGCCACGGG | |
C1⍺AS | TCTTGACCACGTCATCGTTGAG | |
C1βA | CATTTTTCCTCCCACCGCCGTT | |
C1βAS | AAAACTGTGTGTCCCTTCTG | |
RF2S | ATGTATATGTACATCAGGGA | |
RF2AS | CAGGCACATAGCTGGGTACC | |
SNP-1 | GGCTCAACATTGTGTTCCCATTTCAGC | |
SNP-2 | GTGTCAGGAAGACTGGAAGAGGCA | |
P1 | TGTGTATTTTGTAAATATGGTATA | |
P2 | AAGTTAAAGATTTTTATTTTTTATT | |
Cav2 | Cav-2S | ATCTGCAGCCATGCCCTCTTTG |
Cav-2AS | GGGTCCAAGTATTCAATCCTGG | |
C2⍺A | ATGGGGCTGGAGACGGAGAA | |
C2⍺AS | ACTGAAGGCAGAACCATTAGGCA | |
C2βA | TGCGTCCTGTCTCCTCAGCT | |
C2βAS | ACTGAAGGCAGAACCATTAGGCA | |
GAPDH | G2 | CATGTGGGCCATGAGGTCCACCAC |
G3 | AACCATGAGAAGTATGACAACAGC | |
G5 | GAGTCCTTCCACGATACCAAAG |