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Fig. 6 | BMC Cancer

Fig. 6

From: Bidirectional alteration of Cav-1 expression is associated with mitogenic conversion of its function in gastric tumor progression

Fig. 6

Opposite effects of low and high Cav-1 on inhibitory phosphorylation of RAF. a Cav-1 inhibition of EGFR stability. WT-Cav-1- or shCav-1-expressing cells were exposed to EGF (10 ng/ml) and EGFR level was determined using an immunoblot assay. b Effect of Cav-1 on Ras activity. GTP-Ras levels were measured using Ras activity assay. c, d Opposite effects of Cav-1 on EGF-induced MEK1/2 phosphorylation. e, f Effect of Cav-1 on EGF-induced RAF phosphorylation. Stimulatory phosphorylation of A-RAF (P-S299), B-RAF (P-S445) and C-RAF (P-S339) and inhibitory phosphorylation of C-RAF (P-S289/296/301) were detected using antibodies specific to phospho-RAF isoforms

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BMC Cancer

ISSN: 1471-2407

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