Fig. 5

Cav-1 regulation of ERK signaling. a Opposite effects of Cav-1 on ERK1/2 phosphorylation. AGS and MKN1 cells were transfected with WT-Cav-1 or si-Cav-1 and its effect on phosphorylation levels of AKT, ERK1/2, p38, and JNK were examined by an immunoblot assay. b Effect of Cav-1 on EGF-induced phosphorylation of ERK1/2. Cells transfected with WT-Cav-1 or si-Cav-1 were exposed to EGF (10 ng/ml) and phopho-ERK1/2 level was determined. c A dose-associated effect of Cav-1 on EGF-induced ERK1/2 phosphorylation. d Opposite effects of Cav-1 on EGF-induced cell growth. WT-Cav-1- or si-Cav-1-transfected cells were incubated with EGF (10 ng/ml). Data represent means of triplicate assays (Bars, SD)