Fig. 4
Cav-1 effect on tumor cell growth. a Opposite effects of Cav-1 on growth of low-and high-expressing tumor cells. AGS and SNU601 were transfected with 2 μg of WT-Cav-1 expression or empty vector (pcDNA), and MKN1 and MKN28 were transfected with 30 nM of si-Cav-1 or si-Control. Data represent means of triplicate assays (Bars, SD) (*, P < 0.05; **, P < 0.01). b Cav-1 effect on colony forming ability of tumor cells. Cav-1-expressing sublines of AGS and SNU601 and Cav-1-depleted sublines of MKN1 and MKN28 were maintained in the presence of G418 (1600 μg) for 4–6 weeks. c Flow cytometric analysis of Cav-1 effect on cell cycle progression. d [3H]thymidine uptake assay showing opposite effects of Cav-1 on DNA synthesis in AGS and MKN1 cells. e Flow cytometric analysis of Annexin V-positive cells showing no significant effect of Cav-1 on apoptosis. f Flow cytometric analysis of sub-G1 fraction. Cells were exposed to etoposide (15 μM) or 5-FU (15 μM) for 48 h. g Subcellular distribution of WT- and MT-Cav-1 (P132L) proteins. (h-j) Effect of a dominant negative Cav-1 mutant (P132L) on tumor cell growth, colony formation, and apoptosis