MicroRNA expression patterns in canine mammary cancer show significant differences between metastatic and non-metastatic tumours

Table 2 Validation of microarray results in tumour samples

microRNA	microarray p-value	RT-qPCR p-value	Average RT-qPCR repeats, normalized
microRNA	microarray p-value	RT-qPCR p-value	Control	Benign	Malignant non-metastatic	Malignant metastatic
let-7c	< 1e-07	0.02892256	2.066592593	1.551592593	0.885092593	0
miR-26b	< 1e-07	0.00001507	1.56060648	0.04060648	−0.57989352	−1.02131944
miR-26a	< 1e-07	0.00003571	1.11365741	0.50621296	−0.58834259	−1.03152778
miR-30a	3.00E-07	0.00367713	−0.09936889	0.71563112	0.08413112	−0.70039334
miR-148a	3.00E-07	0.04282469	0.32479167	0.55945833	−0.50670833	−0.37754167
miR-29c	5.00E-07	0.00019157	0.79212037	0.30412037	−0.25387963	−0.84236111
miR-30c	2.70E-06	0.00133947	0.2615463	0.59621296	0.0315463	−0.88930556
miR-299	5.40E-06	0.04632324	1.899074074	0.425074074	2.399074074	0
miR-10b	6.00E-06	0.01635353	0.9961408	0.07058524	−0.46735921	−0.59936683
miR-30b	1.74E-05	0.00557426	0.61328913	0.43528913	−0.40171087	−0.6468674

one-way ANOVA p-values for microarray results and RT-qPCR results; RT-qPCRs performed for 10 microRNAs on 47 samples (5 controls, 5 benign tumours, 10 malignant non-metastatic tumours and 27 malignant metastatic tumours)

ISSN: 1471-2407