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Table 3 Dual luciferase reporter assays confirm the interaction between endogenous or exogenous LOCCS and miR-93

From: Long intergenic non-protein-coding RNA 1567 (LINC01567) acts as a “sponge” against microRNA-93 in regulating the proliferation and tumorigenesis of human colon cancer stem cells

 

Endogenous LOCCS

Group

A

B

C

Value

1

2

3

1

2

3

1

2

3

Firefly luciferase (RLU)

360

342

368

283

271

302

410

409

426

Renilla luciferase (RLU)

440

412

440

404

396

388

424

412

418

RLU

0.82

0.83

0.84

0.7

0.68

0.78

0.97

0.99

1.02

Average (mean ± SD)

0.83 ± 0.01*

0.72 ± 0.05*

0.99 ± 0.03

 

Exogenous LOCCS

Group

D

E

F

Value

1

2

3

1

2

3

1

2

3

Firefly luciferase (RLU)

143

135

131

275

279

302

287

296

289

Renilla luciferase (RLU)

340

392

364

376

360

412

396

360

376

RLU

0.42

0.34

0.36

0.73

0.78

0.73

0.72

0.82

0.77

Average (mean ± SD)

0.37 ± 0.04*

0.75 ± 0.03

0.77 ± 0.05

  1. *P < 0.05; RLU relative light unit
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BMC Cancer

ISSN: 1471-2407

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