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Table 3 Dual luciferase reporter assays confirm the interaction between endogenous or exogenous LOCCS and miR-93

From: Long intergenic non-protein-coding RNA 1567 (LINC01567) acts as a “sponge” against microRNA-93 in regulating the proliferation and tumorigenesis of human colon cancer stem cells

  Endogenous LOCCS
Group A B C
Value 1 2 3 1 2 3 1 2 3
Firefly luciferase (RLU) 360 342 368 283 271 302 410 409 426
Renilla luciferase (RLU) 440 412 440 404 396 388 424 412 418
RLU 0.82 0.83 0.84 0.7 0.68 0.78 0.97 0.99 1.02
Average (mean ± SD) 0.83 ± 0.01* 0.72 ± 0.05* 0.99 ± 0.03
  Exogenous LOCCS
Group D E F
Value 1 2 3 1 2 3 1 2 3
Firefly luciferase (RLU) 143 135 131 275 279 302 287 296 289
Renilla luciferase (RLU) 340 392 364 376 360 412 396 360 376
RLU 0.42 0.34 0.36 0.73 0.78 0.73 0.72 0.82 0.77
Average (mean ± SD) 0.37 ± 0.04* 0.75 ± 0.03 0.77 ± 0.05
  1. *P < 0.05; RLU relative light unit