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Fig. 1 | BMC Cancer

Fig. 1

From: Long intergenic non-protein-coding RNA 1567 (LINC01567) acts as a “sponge” against microRNA-93 in regulating the proliferation and tumorigenesis of human colon cancer stem cells

Fig. 1

Generation, proliferative capacity, stem-cell markers of TPSC and their tumorigenicity in nude mice. a Primary cultured human colon cancer cells under light phase-contrast microscopy (×200). b Spheres of human colon cancer cells in the SFM suspension culture system. c Expression of CD133, CD166 and CD44 stem cell surface markers in colon cancer spheroid cells. Flow cytometry dot plots showing that colon cancer sphere cells expressed high levels of CD166 and CD44 in SFM. Cancer sphere cells incubated with FITC-conjugated anti-CD44 and PE-conjugated anti-CD166 monoclonal antibodies. d Growth curves of TPSC and PCC. e Colony formation rates of TPSC and PCC. f Tumor xenografts in nude mice. The volume of tumors generated by 1 × 105 TPSC and PCC 63 days after injection. Left: TPSC; Right: PCC. g Expression of stem-cell markers in human colon cancer spheroid cells. Each experiment was performed in triplicate

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BMC Cancer

ISSN: 1471-2407

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