Fig. 3

Multiple evidence sources makes GBP1 arise as potential target for TNBC. a GBP1 is more highly expressed in TNBC than in non-TNBC and normal tissues (left) and in TNBC versus non-TNBC cell lines (right). FDR values were obtained from the DESeq2 comparisons. b Cartoon representation of the human GBP1 protein structure (PDB ID 1DG3), displaying the 5 highest-scoring potential small molecule binding pockets according to PockDrug [54]. c MS evaluation of GBP1 protein level in Non-TNBC and TNBC samples. P-Value and FDR value were calculated with limma. d Seven microarray datasets external-to-our-pipeline analysis confirmed GBP1 upregulation in TNBC versus non-TNBC tissues. FDR values were derived from limma comparisons. (e, lower) GBP1 gene scheme denoting the open-sea probe cg12054698 location within the exon 1. (e, upper) Methylation status (as defined by M-values) for the cg12054698 in Normal, Non-TNBC and TNBC samples, showing hypomethylation in TNBC. FDR values calculated with limma. As for all the displayed box-plots, log₂-transformed upper-quantile values were used, with the whiskers extending to half of the interquartile range. Gray circles denote each sample. Notches, when present, denotes the 95% confidence intervals of the median