Skip to main content
Fig. 3 | BMC Cancer

Fig. 3

From: MicroRNA-195 acts as an anti-proliferative miRNA in human melanoma cells by targeting Prohibitin 1

Fig. 3

PHB1 overcomes the anti-proliferative effect of miRNA-195. (a) Proliferation assay based on nuclear counting per mm2. UACC-62 melanoma cells were transfected with either miR-control or miR-195 (25 nM) and observed for five days after transfection. (b) To conduct rescue experiments, UACC-62 melanoma cells were stably expressing either ORF-PHB1 or pcDNA3.1-EV. Cells were then transfected with either miRNA-mimics control or miR-195 mimics. After transfection, the proliferation rate was measured for six days and the results showed that cells transfected with transgenic PHB1 overcome the suppressive effect of miR-195 (green line) compared to pcDNA3.1-EV cells (pink line). Representative examples of at least three independent experiments are reported

Back to article page