Fig. 5

Spontaneous and acute UVB-irradiated skin from trigenic mice exhibit altered expression of regulators of signaling. a Immumnoblotting analyses of non-UV irradiated TAN skin samples showed modest upregulation in pAKT, significant upregulation of p21 and Cyclin D1 and a modest decrease in BAX expression in the Rxrα ^ep−/− mutant mice relative to the controls in the untreated group. b Western blot analysis of single UVB treated groups of control and trigenic Rxrα ^ep−/− mutant mice exhibiting significant upregulation of pAKT, p21 and Cyclin D1 expression and a significant decrease in BAX expression in the trigenic mice relative to the controls. Equal loading was confirmed by β-actin. 2–3 biological replicates for each group are shown for all immunoblots