Fig. 4
Enhanced metastasis to draining lymph nodes in trigenic RXRα ep−/−|Tyr-NRAS Q61K|Cdk4 R24C/R24C mice relative to Rxrα L2/L2 control mice. a, b Excised draining lymph nodes from RXRαL2/L2/Tyr-NRASQ61K/Cdk4R24C/R24C and RXRαep−/−/TyrNRAS Q61K/Cdk4R24C/R24C mice at age (a) 12 months for untreated mice and (b) 6 months for single UVB treated mice. (c, d) Histological analyses of draining lymph nodes from Tyr-NRASQ61K/Cdk4R24C mice with functional and ablated Rxrα in (c) no UVB treatment as well as the (d) acute UVB treated mice. e, f Chromogenic IHC for melanocyte-specific marker TYRP1 (brown). More positive staining overall is observed in RXRαep−/−/TyrNRAS Q61K/Cdk4R24C/R24C LNs as opposed to their relative RXRαL2/L2 control LNs in both the UVB untreated and treated groups. Hematoxylin (purple) was used as a nuclear counterstain. Scale bar =100 μm