Fig. 3

Screening studies of breast cancer cell lines in microfluidic culture. a For paclitaxel and olaparib studies, HCC-1937 were seeded 3D in the OrganoPlate® and 2D on tissue culture grade plastics 96-well flat bottom plates, cultured for 1 day before 72 h exposure with compounds at the specified concentrations. Viability (as % of total cells) was quantified using an optimized RealTime-Glo™ cell viability assay. (B) MDA-MB-231 and MDA-MB-453 were seeded for 24 h similarly and exposed to cisplatin at various concentrations for 48 h. Symbols: * P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001, **** P ≤ 0.0001 using Tukey’s multiple comparison test (See supplementary documents for p-values and more detailed analysis)