Fig. 6

EGF treatment stimulates anterograde lysosome trafficking, protease secretion, and invasion in 3D culture. a DU145 cells were cultured on Matrigel in the presence or absence of 100 ng/mL EGF. Cells were fixed and stained for DAPI (blue), actin (red), and LAMP-1 (green). Representative 63X confocal images are shown, N = 3. b DU145 cells were plated in Matrigel and 25 μM DQ-Collagen IV for 48 h. Cells were then treated with 25 μM EIPA, 10 μM SB203580, or stimulated with 100 ng/mL EGF for 48 h. Cells were then fixed and stained with phalloidin (red). Cells and cleaved DQ-Collagen IV (green) were imaged using confocal microscopy. Representative 40X images are shown and scale bar represents 30 μm, N = 3. c Quantification of extracellular DQ-collagen IV fluorescence from panel B. Error bars represent standard error of the mean. * = p < 0.01 compared to control