Fig. 5

p38 inhibition does not block EGFR activation or signaling. a DU145 cells were treated with 10 μM SB203580 or 0.1% DMSO for 30 min prior to stimulation with varying concentration of EGF for 10 min. Whole cell lysates were collected and assessed by western blot. b Cells were treated with the indicated concentrations of SB203580 and EGF for 16 h. Cells were fixed and stained with phalloidin. Representative 10X images are shown, N = 3. c Cells were treated with the indicated concentrations of SB203580 or EGF for 16 h. Cells were fixed and stained for LAMP-1 (red), phalloidin (green), and DAPI (blue), N = 3. Scale bar represents 30 μm. d Quantification of lysosome distribution of 25 cells per treatment. Error bars represent standard error of the mean. * = p < 0.05 vs. DMSO