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Fig. 2 | BMC Cancer

Fig. 2

From: Lysosome trafficking is necessary for EGF-driven invasion and is regulated by p38 MAPK and Na+/H+ exchangers

Fig. 2

EGF-stimulated lysosome trafficking is due to EGFR activation and not crosstalk with c-Met. a DU145 cells were treated with 100 ng/mL EGF or 33 ng/mL HGF for 16 h then stained for LAMP-1 (red), actin (green) and DAPI (blue). Scale bar represents 30 μm, N = 3. b Quantification of lysosome distribution for 25 cells; mean values are shown. * = p < 0.05 compared to control. c DU145 cells were treated for 2 h with 10 μM AG1478 or SU11274 prior to stimulation with 100 ng/mL EGF or 33 ng/mL HGF for 10 and 30 min, respectively. Total protein lysates were harvested and analyzed by western blot. d Cells were stimulated with 33 ng/mL HGF for 30 min or 100 ng/mL EGF over time. Total protein lysates were harvested and analyzed via western blot. e DU145 cells were treated with 10 μM AG1478 or 5 μM SU11274, for 2 h then stimulated with 100 ng/mL EGF for 16 h. Cells were then fixed and stained for LAMP-1 (red), DAPI (blue), and phalloidin (green). Scale bar represents 30 μm, N = 3. f Quantification of lysosome distribution of 25 cells per condition. Error bars represent standard error of the mean. * = p < 0.05 compared to DMSO control

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