Fig. 4
From: 3,6-Dihydroxyflavone regulates microRNA-34a through DNA methylation
The methylation status of miR-34a and TET1 promoters. a The methylation status of miR-34a promoter in MDA-MB-231 cells with 3,6-DHF (20 μM) treatment for 24 h, or transfecting TET1 siRNA before 3,6-DHF (20 μM) treatment for 24 h. or transfecting pcDNA3/Myc-DNMT1 before 3,6-DHF (20 μM) treatment for 24 h. b The level of the DNA methylation of miR-34a promoters in MDA-MB-231 cells as determined by qPCR according to Fig. 4a. c The methylation status of the TET1 promoter in MDA-MB-231 cells after 3,6-DHF (20 μM) treatment for 24 h, or transfecting of pcDNA3/Myc-DNMT1 before 3,6-DHF (20 μM) treatment. d The level of the DNA methylation of TET1 promoters in MDA-MB-231 cells as determined by qPCR according to Fig. 4c. Methylation status was detected by MSP; methylation levels are also detected with qPCR. M: methylated; U: unmethylated. The data are presented as the mean ± SD (n = 3). *P < 0.05 compared with the control or compared with 0 μM