Fig. 3
From: 3,6-Dihydroxyflavone regulates microRNA-34a through DNA methylation
3,6-DHF reactivates the expression of tumor suppressor miR-34a through increasing TET1 level in breast cancer cells. a Western blots showing levels of TET1 in MDA-MB-231 cells after transfecting TET1 siRNA. b The effect of 3,6-DHF (20 μM) on the levels of TET1 in MDA-MB-231 cells after transfecting TET1 siRNA, detected by Western blotting. c The effect of 3,6-DHF (0, 20 μM) on the levels of miR-34a in MDA-MB-231 cells after transfecting TET1 siRNA or pcDNA3/Myc-DNMT1(DNMT1) as detected by qRT-PCR. The data are presented as the mean ± SD (n = 3). * P < 0.05, ** P < 0.01 compared with the control