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Table 5 Transcriptional activity of the regulatory region in target cells

From: Cytotoxicity of replication-competent adenoviruses powered by an exogenous regulatory region is not linearly correlated with the viral infectivity/gene expression or with the E1A-activating ability but is associated with the p53 genotypes

  Transcriptional activity (% activity of SV40 T antigen)
Cells MK Sur COX-2
Pancreatic carcinoma
 AsPC-1 345 ± 59.9 75.6 ± 2.9 212 ± 11.5
 PANC-1 224 ± 3.6 69.8 ± 4.28 243 ± 11.4
 BxPC-3 454 ± 49.1 67.8 ± 10.6 363 ± 86.5
 MIA-PaCa-2 182 ± 22.4 135 ± 7.0 783 ± 27.6
Esophageal carcinoma
 TE-1 400 ± 99.0 162 ± 13.6 752 ± 71.9
 TE-2 375 ± 7.9 610 ± 136.5 625 ± 28.1
 TE-10 339 ± 26.9 906 ± 34.9 431 ± 59.5
 TE-11 702 ± 30.2 6770 ± 526.0 410 ± 128.0
 YES-2 314 ± 20.2 1790 ± 156.0 351 ± 27.1
 YES-4 187 ± 2.6 310 ± 13.2 282 ± 18.5
 YES-5 745 ± 47.7 395 ± 11.2 160 ± 12.5
 YES-6 540 ± 7.4 3110 ± 96.8 417 ± 31.4
 T.Tn 199 ± 32.0 2410 ± 233.0 126 ± 10.2
Mesothelioma
 NCI-H2452 153 ± 45.7 35.0 ± 5.0 291 ± 72.4
 NCI-H2052 189 ± 4.5 38.6 ± 1.0 233 ± 10.1
 NCI-H226 112 ± 4.2 7.2 ± 0.3 381 ± 61.2
 NCI-H28 348 ± 68.2 173 ± 62.1 250 ± 38.2
 MSTO-211H 168 ± 18.4 134 ± 7.3 141 ± 14.0
  1. Cells were transfected with plasmid vector DNA containing a regulatory region linked with the luciferase gene and the transcriptional activity was expressed as a percent luciferase activity of the SV40 T antigen. Three histological types were respectively examined with SV40 T antigen as a control. Averages and SEs are shown (n = 3)