Cytotoxicity of replication-competent adenoviruses powered by an exogenous regulatory region is not linearly correlated with the viral infectivity/gene expression or with the E1A-activating ability but is associated with the p53 genotypes

Table 5 Transcriptional activity of the regulatory region in target cells

	Transcriptional activity (% activity of SV40 T antigen)
Cells	MK	Sur	COX-2
Pancreatic carcinoma
AsPC-1	345 ± 59.9	75.6 ± 2.9	212 ± 11.5
PANC-1	224 ± 3.6	69.8 ± 4.28	243 ± 11.4
BxPC-3	454 ± 49.1	67.8 ± 10.6	363 ± 86.5
MIA-PaCa-2	182 ± 22.4	135 ± 7.0	783 ± 27.6
Esophageal carcinoma
TE-1	400 ± 99.0	162 ± 13.6	752 ± 71.9
TE-2	375 ± 7.9	610 ± 136.5	625 ± 28.1
TE-10	339 ± 26.9	906 ± 34.9	431 ± 59.5
TE-11	702 ± 30.2	6770 ± 526.0	410 ± 128.0
YES-2	314 ± 20.2	1790 ± 156.0	351 ± 27.1
YES-4	187 ± 2.6	310 ± 13.2	282 ± 18.5
YES-5	745 ± 47.7	395 ± 11.2	160 ± 12.5
YES-6	540 ± 7.4	3110 ± 96.8	417 ± 31.4
T.Tn	199 ± 32.0	2410 ± 233.0	126 ± 10.2
Mesothelioma
NCI-H2452	153 ± 45.7	35.0 ± 5.0	291 ± 72.4
NCI-H2052	189 ± 4.5	38.6 ± 1.0	233 ± 10.1
NCI-H226	112 ± 4.2	7.2 ± 0.3	381 ± 61.2
NCI-H28	348 ± 68.2	173 ± 62.1	250 ± 38.2
MSTO-211H	168 ± 18.4	134 ± 7.3	141 ± 14.0

Cells were transfected with plasmid vector DNA containing a regulatory region linked with the luciferase gene and the transcriptional activity was expressed as a percent luciferase activity of the SV40 T antigen. Three histological types were respectively examined with SV40 T antigen as a control. Averages and SEs are shown (n = 3)

ISSN: 1471-2407