Cytotoxicity of replication-competent adenoviruses powered by an exogenous regulatory region is not linearly correlated with the viral infectivity/gene expression or with the E1A-activating ability but is associated with the p53 genotypes

Table 4 Correlation between Ad-mediated cytotoxicity and Ad infectivity/gene expression

Tumor type	Infectivity^b	Correlation coefficient	P value
Ad-mediated cytotoxicity^a
Pancreatic carcinoma
Ad5/MK	Ad5/GFP	0.77	0.22
Ad5/Sur	Ad5/GFP	0.05	0.95
Ad5/COX-2	Ad5/GFP	0.94	0.06
AdF35/MK	AdF35/GFP	−0.89	0.11
AdF35/Sur	AdF35/GFP	0.31	0.69
AdF35/COX-2	AdF35/GFP	0.14	0.86
Esophageal carcinoma
Ad5/MK	Ad5/GFP	−0.15	0.70
Ad5/Sur	Ad5/GFP	−0.52	0.15
Ad5/COX-2	Ad5/GFP	−0.22	0.56
AdF35/MK	AdF35/GFP	−0.36	0.34
AdF35/Sur	AdF35/GFP	−0.28	0.47
AdF35/COX-2	AdF35/GFP	−0.53	0.13
Mesothelioma
Ad5/MK	Ad5/GFP	−0.57	0.31
Ad5/Sur	Ad5/GFP	−0.65	0.24
Ad5/COX-2	Ad5/GFP	−0.18	0.82
AdF35/MK	AdF35/GFP	0.10	0.87
AdF35/Sur	AdF35/GFP	0.07	0.91
AdF35/COX-2	AdF35/GFP	0.15	0.82

^aCytotoxicity data are expressed as IC₅₀ values of respective cells, 4 pancreatic, 9 esophageal carcinoma and 5 mesothelioma cells as shown in Table 1
^bInfectivity data are percentages of GFP-positive cells infected at Ad5/GFP or AdF35/GFP at 30 MOI as shown in Table 2

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