Cytotoxicity of replication-competent adenoviruses powered by an exogenous regulatory region is not linearly correlated with the viral infectivity/gene expression or with the E1A-activating ability but is associated with the p53 genotypes

Table 2 Infectivity/gene expression of Ad5/GFP and AdF35/GFP, and expression levels of CAR and CD46 on target cells

	Infectivity tested at MOI = 30		Receptor expression
Cells	Ad5/GFP	AdF35/GFP	CAR	CD46
Cells	(% positive cells^a)		(% mean fluorescence intensity^b)
Pancreatic carcinoma
HEK293	98.98 ± 0.11	99.47 ± 0.11	100	100
AsPC-1	8.47 ± 0.27	25.32 ± 0.57	71.6	182.4
PANC-1	7.66 ± 0.50	28.35 ± 0.41	65.5	175.7
BxPC-3	19.48 ± 0.62	68.95 ± 0.45	26.8	231.1
MIA-PaCa-2	5.89 ± 0.54	65.41 ± 0.68	2.0	62.6
Esophageal carcinoma
HEK293	87.20±0.52	78.05±0.70	100	100
TE-1	8.06±1.17	53.47±0.10	11.5	321.5
TE-2	0.79±0.14	10.15±0.44	33.7	160.2
TE-10	16.15±0.52	35.33±0.67	25.9	99.7
TE-11	22.86±0.53	42.82±0.74	39.1	290.2
YES-2	5.09±1.29	51.54±0.36	0.3	131.7
YES-4	27.18±0.16	61.23±0.07	47.2	135.2
YES-5	22.18±0.32	69.97±0.89	27.0	176.3
YES-6	16.59±0.25	27.63±0.17	53.1	120.4
T.Tn	0.49±1.00	21.60±0.15	14.3	208.9
Mesothelioma
HEK293	93.7±0.1	93.8±0.4	100	100
NCI-H2452	49.8±0.3	75.3±0.6	35.6	117.6
NCI-H2052	7.4±0.3	81.5±0.5	0.7	174.7
NCI-H226	78.5±1.2	81.9±1.5	84.0	164.7
NCI-H28	85.5±0.4	95.1±0.2	17.8	150.4
MSTO-211H	8.7±0.3	67.9±0.9	2.3	56.0

Cells infected with Ad5/GFP or AdF35/GFP at 30 MOI were analyzed for the fluorescence intensity with flow cytometry. ^aPositively stained cells were defined as those that showed fluorescence greater than the brightest 5% of uninfected cells. Averages and the SEs are shown (n = 3). ^bCAR and CD46 expression levels were determined with flow cytometry and are expressed with arbitrary unit. The intensity is expressed as a percentage of that of HEK293 cells. Three tumor types were respectively examined with HEK293 cells as a control

ISSN: 1471-2407