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Table 2 Infectivity/gene expression of Ad5/GFP and AdF35/GFP, and expression levels of CAR and CD46 on target cells

From: Cytotoxicity of replication-competent adenoviruses powered by an exogenous regulatory region is not linearly correlated with the viral infectivity/gene expression or with the E1A-activating ability but is associated with the p53 genotypes

  Infectivity tested at MOI = 30 Receptor expression
Cells Ad5/GFP AdF35/GFP CAR CD46
(% positive cellsa) (% mean fluorescence intensityb)
Pancreatic carcinoma
 HEK293 98.98 ± 0.11 99.47 ± 0.11 100 100
 AsPC-1 8.47 ± 0.27 25.32 ± 0.57 71.6 182.4
 PANC-1 7.66 ± 0.50 28.35 ± 0.41 65.5 175.7
 BxPC-3 19.48 ± 0.62 68.95 ± 0.45 26.8 231.1
 MIA-PaCa-2 5.89 ± 0.54 65.41 ± 0.68 2.0 62.6
Esophageal carcinoma
 HEK293 87.20±0.52 78.05±0.70 100 100
 TE-1 8.06±1.17 53.47±0.10 11.5 321.5
 TE-2 0.79±0.14 10.15±0.44 33.7 160.2
 TE-10 16.15±0.52 35.33±0.67 25.9 99.7
 TE-11 22.86±0.53 42.82±0.74 39.1 290.2
 YES-2 5.09±1.29 51.54±0.36 0.3 131.7
 YES-4 27.18±0.16 61.23±0.07 47.2 135.2
 YES-5 22.18±0.32 69.97±0.89 27.0 176.3
 YES-6 16.59±0.25 27.63±0.17 53.1 120.4
 T.Tn 0.49±1.00 21.60±0.15 14.3 208.9
Mesothelioma
 HEK293 93.7±0.1 93.8±0.4 100 100
 NCI-H2452 49.8±0.3 75.3±0.6 35.6 117.6
 NCI-H2052 7.4±0.3 81.5±0.5 0.7 174.7
 NCI-H226 78.5±1.2 81.9±1.5 84.0 164.7
 NCI-H28 85.5±0.4 95.1±0.2 17.8 150.4
 MSTO-211H 8.7±0.3 67.9±0.9 2.3 56.0
  1. Cells infected with Ad5/GFP or AdF35/GFP at 30 MOI were analyzed for the fluorescence intensity with flow cytometry. aPositively stained cells were defined as those that showed fluorescence greater than the brightest 5% of uninfected cells. Averages and the SEs are shown (n = 3). bCAR and CD46 expression levels were determined with flow cytometry and are expressed with arbitrary unit. The intensity is expressed as a percentage of that of HEK293 cells. Three tumor types were respectively examined with HEK293 cells as a control