Fig. 4

mRNA levels for IGFBP5 and IGFBP2 are biomarkers for bladder tumor cells sensitivity to IGF1R kinase inhibitor, AEW541. a Heat map of the IGF receptors, ligands, and binding proteins mRNA levels in regards to sensitivity to AEW41 in 13 bladder tumor derived cell lines. Data were extracted from CCLE database; b Effect of a blocking antibody against IGF1R and of IGF1R siRNA on the viability of RT112 cells. IGF1R knockdown 72 h after transfection with a control or anti-IGF1R siRNA was assessed by western blotting (inset). The effect of the siRNA on cell viability was assessed in MTT assays. The effect of the anti-IGF1R blocking antibody was assessed after 72 h, in MTT assays; c Spearman’s coefficients for the correlations between the sensitivity to AEW541 and mRNA levels for IGF receptors, ligands, and binding proteins, in 13 cell bladder tumor derived cell lines. Data were extracted from CCLE database