Fig. 8

TRAP-dependent proliferation and migration are mediated via the TGFβ2/TβR and CD44. Functional analysis after treatment with a blocking antibody anti-TGFβ2 (0.25 μg/mL, a-c, n = 6), the small molecule inhibitor LY2109761 against TGFβ receptor type 1/type 2 kinase activity (2 μM, d-f, n = 4) or with a blocking antibody anti-CD44 (10 μg/mL, g-i, n = 3) and respective controls. Proliferation was assessed after 24 h treatment in complete medium (a, d, g). Live cell imaging was performed over 30 h in serum-free medium containing blocking antibody or small chemical inhibitor and wound migration velocity (b, e, h) and migration curves (c, f, i) are compared. Statistical comparison was performed on biological replicates by ANOVA test. Groups are generally compared to their respective controls (ctrl, untreated) or indicated with brackets and significance is annotated with an asterisk (*). ns = non-significant. “n=” indicates the number of replicates