Fig. 7

TRAP overexpression upregulates TGFβ-pathway associated proteins. Expression of TGFβ2, respectively after 24 h treatment with the small molecule TRAP inhibitor 5-PNA (200 μM) or respective control in complete medium. Quantification of Immunocytochemistry (a, b, n = 3) and Western blotting (c, d, n = 4). One representative image of immunocytochemistry staining (a, TGFβ2, green; Hoechst, blue) or blot (c, TGFβ2, 48 kDa; normalization control β-Actin 42 kDa) is shown. Quantification was performed by analysis of fluorescent intensities per cell for immunocytochemistry (b) and by densitometry for western blot analysis (d). Statistical comparison was performed on biological replicates by or t-test (Fig. 7 b) or ANOVA test (Fig. 7 d). Groups are generally compared to their respective controls (ctrl, untreated) or indicated with brackets and significance is annotated with an asterisk (*). ns = non-significant. “n=” indicates the number of replicates