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Fig. 5 | BMC Cancer

Fig. 5

From: Tartrate-resistant acid phosphatase (TRAP/ACP5) promotes metastasis-related properties via TGFβ2/TβR and CD44 in MDA-MB-231 breast cancer cells

Fig. 5

Proteomics and phosphoproteomics profiling of TRAP knockdown cells. Experimental workflow applied to perform standard proteomics analysis of TRAP3high MDA-MB-231 cells transfected with a shRNA with scrambled sequence (scr, n = 4) or with two different shRNAs targeting TRAP (sh2 and sh3 + 4, n = 3 each) (a). Protein extracts from each condition were digested to peptides with trypsin, labeled with Tandem Mass Tags (TMT), pooled, fractionated by HiRIEF using a wide-range (pH 3–10) IPG strip and analyzed by LC-MS. Heatmap representing complete linkage hierarchical clustering based on Euclidian distance of protein ratios measured in the proteomics analysis (b). Ratios are represented for each sample relative to the average of the scrambled shRNA samples (b). Row color-coding indicates significantly regulated proteins (red colored), defined by a log2(ratio) of more than −3/+3 MAD away from the median and t-test p-value <0.01. “n=” indicates the number of replicates

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