Skip to main content
Fig. 4 | BMC Cancer

Fig. 4

From: Spatial and temporal epithelial ovarian cancer cell heterogeneity impacts Maraba virus oncolytic potential

Fig. 4

Extracellular factors do not impart sensitivity to MRBV. a MRBV-sensitive iOvCa147-F8, and MRBV-resistant iOvCa147-B3 and iOvCa147-G4 cells were seeded at 500,000 cells per well of a 6-well plate. A549 human lung adenocarcinoma cells served as a type I IFN response positive control cell line. The following day, cells were infected with MRBV at an MOI of 1 or UV-inactivated MRBV for 6 h. RNA was isolated to perform qRT-PCR using human-specific IFNB1 primers and SYBR Green detection; GAPDH served as a normalization control. (****, p < 0.0001, as determined by one-way ANOVA and Dunnett’s posthoc test). b Cells were seeded at 10,000 cells per well of a 96-well plate. After 16 h, fresh media was either (i) replaced, (ii) conditioned media was swapped, or (iii) left unchanged between iOvCa147-F8 and iOvCa147-G4 cells. Cells were then immediately infected with MRBV at an MOI of 0.1 for 1 h followed by media change. CellTiter-Glo® assays were performed for cell viability 48 h post-infection. c iOvCa147-F8 and iOvCa-G4 cells were seeded at 10,000 cells per well of a 96-well plate. After 16 h, cells were infected with MRBV at an MOI of 0.1 for 1 h followed by media change. At 12 h, fresh media was either (i) replaced, or (ii) conditioned media was swapped between iOvCa147-F8 and iOvCa-G4 cells, or (iii) left unchanged. CellTiter-Glo® assays were performed for cell viability 48 h post-infection. (Letters indicate whether there is a statistically significant difference (p < 0.05) among conditions as determined by one-way ANOVA and Tukey’s posthoc test)

Back to article page