Fig. 2

Intratumoral heterogeneity impacts MRBV oncolytic efficacy in vitro. a. Seven subcloned derivatives from iOvCa147 were generated through limiting dilution culturing and single-cell expansion to create homogeneous cell lines from the original heterogeneous cell line population. Resultant cells were seeded at 10,000 cells per well of a 96-well plate and infected 24 h post-seeding. Cells were assayed for viability using CellTiter-Glo at 72 h post-infection. (**, p < 0.01; ****, p < 0.0001, as determined by one-way ANOVA and Dunnett’s posthoc test). b. Images of MRBV-infected cells were captured at 72 h post-infection using bright-field and fluorescence microscopy at 50× original magnification