Fig. 5From: Positive regulation of prostate cancer cell growth by lipid droplet forming and processing enzymes DGAT1 and ABHD5Immunoblotting and immunofluorescence experiments depicting pathways after ABHD5 and DGAT1 siRNA treatment leading to apoptosis and autophagy respectively: a and b- Immunoblotting assay using different antibodies after siRNA transfection with NT (non-target), DGAT1 and ABHD5 siRNA. GAPDH has been used as an internal control. A representative experiment is shown out of the three repeats. c Immunofluorescence assay showing LC3 punctate staining after DGAT siRNA treatment representing autophagy. DAPI (blue)-nucleus and TRITC (red)-LC3. Bar is 50 μmBack to article page