Fig. 1

Comparative expression of fatty acid metabolism regulating genes in LNCaP and PBMCs. a Screening for fatty acid metabolism genes which are differentially expressed in LNCaP and PBMCs was done using western blot analysis; the genes differentially expressed are shown. GAPDH is used as an internal control. The experiment was repeated three times and a representative experiment is shown. HeLa and differentiated OP9 cell extracts have been used as positive control for DGAT2, perilipin and HSL expression. b Histogram plot showing comparative fold expressions of ABHD, ACAT, ATGL and DGAT1 between LNCaP and PBMCs. The fold change is calculated by taking base levels expressed in LNCaP cell line as 1 after GAPDH calibration and is an average of three separate experiments, (*)-(P ≤ 0.05). c Effect of plasma treatment on the expression levels of ABHD5, ACAT1, ATGL and ABHD5. Western blots were performed after 50% plasma treatment for 12 h. on both PBMCs and LNCaP cells. Out of the three experimental repeats a representative experiment is shown. Fold changes mentioned in the results section are an average of all the experiments