Fig. 2

VEGF secretion and VEGFR2 expressions were elevated in HCT116 cells under hypoxia. a VEGFA secretion of HCT116 cells under hypoxia or normoxia were determined by ELISA analysis. The data are shown as mean ± SD, n = 3 in triplicate experiments. b Protein expressions of CD31, CD34, and VE-cadherin in HCT116 cells under hypoxia or normoxia were determined by western blotting (left) and immunofluorescent staining analysis (right). Representative data of three experiments are shown. c Protein expressions of VEGFR1, VEGFR2, and VEGFR3 in HCT116 cells under hypoxia or normoxia were determined by western blotting. Representative data of three experiments are shown