Fig. 1

Endothelial-inducing conditioned medium induced expressions of CD31, CD34, and VE-cadherin and promoted HCT116 cells to form tube-like structures. a Protein expressions of CD31, CD34, and VE-cadherin in HT29, SW480 or HCT116 after cultured for 5, 10 or 15 days in endothelial-inducing conditioned medium were determined by western blotting analysis. Representative data of three experiments are shown. b The tube-like structures formed by HT29, SW480 or HCT116 cells in endothelial-inducing conditioned or normal 3D culture (left). Black arrows indicate the typical tube-like structures. Scale bar: 100 μm; Quantitative analysis of the mean number of tube-like structures and shown as mean ± SD (right)