Fig. 3
From: EMX2 gene expression predicts liver metastasis and survival in colorectal cancer
a qRT-PCR revealing EMX2 transcript expression in two different colorectal cancer cell lines (DLD1, CaCo2). b Semiquantitative analysis of immunoblotting revealing residual EMX2 expression after control-transfection versus siRNA-mediated EMX2 knockdown in CaCo2 cells and c Ad-Null versus Ad-EMX2-transfected DLD1 cells. Bars indicate mean ± SEM. d Transwell migration assay revealing enhanced migratory potential of CaCo2 cells upon siRNA-mediated knockdown of EMX2 compared to control-transfected (scrambled) cells. Restoration of EMX2 expression in e DLD1 cells using an adenoviral delivery system resulted in attenuated migration. Ad-Null and Ad-EMX2 represent the empty adenoviral vector control and the adenoviral vector containing EMX2 cDNA, respectively. Bars indicate mean ± SEM of three independent experiments (* P < 0.05)