Fig. 8

Cell migration data. Cell migration was analyzed using the three-dimensional collagen matrix migration assay in combination with computer-assisted cell-tracking. a The locomotor activity of the cell population is displayed as a box plot. b The time active of all single cells analyzed in this study is shown as a bar chart. Non-migrating cells possess a time active of 0%. With the exception of the M13HS-7 hybrid clone cells, each cell line responded to EGF with an increased locomotor activity. Thereby, the time active data reveal that the EGF-induced cell migration was attributed to an increased number of moving cells and a prolonged time of active movement. Shown are the means of at least three independent experiments. Statistical analysis was performed using a two-tailed Mann-Whitney U test. ** = p < 0.01; *** = p < 0.001