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Fig. 6 | BMC Cancer

Fig. 6

From: Hybrid clone cells derived from human breast epithelial cells and human breast cancer cells exhibit properties of cancer stem/initiating cells

Fig. 6

Confocal laser scanning microscopy of intracellular SOX9 and SLUG localization within mammospheres. Mammospheres were grown for 10 days, fixed, permeabilized and stained for SOX9, SLUG and DNA. In accordance with Fig. 2, white arrows indicate cells with a nuclear co-localization of SOX9 and SLUG. Green arrowheads indicate cells with nuclear SOX9 and cytosolic SLUG. Due to the size of the mammospheres, the inner regions remain unstained. Nonetheless, cells with a nuclear co-localization of SOX9 and SLUG could be found in the periphery of the mammospheres. The white square indicates areas that were further analyzed at a higher magnification. Bar = 50 μm

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