Fig. 4
M13HS hybrid clone cells exhibit an increased colony formation capacity. Cells (2×102 per well) were seeded in 6-well plates and were cultivated in complete media for 10–14 days. Growing colonies were stained with crystal violet solution. a Shown is the mean relative colony formation capacity of three independent experiments in relation to that of the M13SV1-EGFP-Neo human breast epithelial cells, which was set to 1. Statistical analysis was performed using an unpaired two-tailed Student’s t-test: * = p < 0.05, ** = p < 0.01, *** = p < 0.001 (vs. M13SV1-EGFP-Neo cells); † = p < 0.05, †† = p < 0.01, ††† = p < 0.001 (vs. HS578T-Hyg cells). b Shown are representative images of colonies derived from the indicated cell lines