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Fig. 1 | BMC Cancer

Fig. 1

From: Live-cell calcium imaging of adherent and non-adherent GL261 cells reveals phenotype-dependent differences in drug responses

Fig. 1

GL261 phenotype is dependent on culture conditions. GL261 cells grow adherently when cultured in media that contains serum. Cells cultured in serum-free media supplemented with EGF, FGF and B-27 grow as detached free-floating aggregates (neurospheres). When experimental manipulations involve acute drug treatments delivered to the media, live-cell fluorescent imaging of neurospheres presents a technical challenge as any treatment delivered to the culture medium causes cell movement

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